首先。什么是FTIR成像?
In an FTIR image (often called chemical image), each pixel is composed of an entire IR spectrum. These images allow a very detailed and spatially resolved chemical analysis based on a sample’s spectroscopic properties. By interpreting this often massive amount of data, sample properties like chemical structure or composition can be visualized by rendering false color images.
FTIR成像在哪里使用?
这对于许多行业来说是非常有价值的,并且用于研发以及QC和根本原因分析。现实生活中的例子是评估聚合物涂料均匀性,药物中API的分布或生物组织中生物标志物的研究。
从automotive industries to pharmaceutical formulation development to life-science and microplastic particle analysis. The applicability of FTIR imaging is almost endless and our experts at Bruker are always ready to claim unchartered land and develop exciting, new solutions.
Figure 1: Chemical FTIR Image of a pharmaceutical tablet.
A specific color was assigned to each component.
How are FTIR images created?
通常,单点,线数组和焦平面阵列(FPA)测量是生成此类图像的选择。当然,性能在这些不同的方法上有所不同。
In single-point imaging, each measurement point of a defined grid is approached and measured. This means a high investment of time, whereas highly automatic microscopes reduce this effort.
在线阵列探测器中,单个检测器元件以串联(例如1x8)排列,并记录一条在测量后一起“缝合”的光谱线,以形成化学图像。
关于FPA探测器的主导地位。
Focal-plane array detectors are composed of a set of infrared light-sensitive pixels in the focal plane of a lens - just like a digital camera. This way it is possible to image the desired field of view without the need for time-consuming scanning procedures.
无论样品结构如何,数据都与视觉图像完美对齐。因此,自然而然地,FPA探测器在速度和精度方面很容易超过经典的连续单点或线阵列测量值。
布鲁克最近扩展并增强了其FPA成像显微镜阵容,并引入了LUMOS II FTIR microscope。It is equipped with a 32x32 pixel line array detector and collects an impressive number of 1024 spectra per scan.
And this brings us back to our first question: why应该you (re)consider FTIR imaging? Because the LUMOS II is a game changer, and here is the reason:
>900 spectra per second, at 5 µm resolution.
听起来很棒?看起来令人印象深刻!我们录制了一个简短的视频,显示了切割微型组织的实时分析。使用Lumos II和我们的Opus光谱软件的最新版本进行的测量。以每秒900多个光谱记录图像,以5 µm像素分辨率和1 x 1mm²的面积记录。
FTIR Imaging by FPA and LUMOS II
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